CURRENT RESEARCH TOPICS
1) Transcriptional Regulation
of Polycystic Kidney Disease-1 (PKD-1) and HFE genes
2) Nanoparticles: A) Low cost,
large scale production
B)
Antimicrobial effect
C)
Cytotoxicity
PUBLICATIONS
1.
C Pettegrew, Z Dong, MR Islam (2009) A simple method of detection
and quantification of sugars using silver nanoparticles;
submitted
to Int. J. Nanoscience
2.
A Swatek, Z Dong, J Shaw, MR Islam (2009) Self-assembly of Silver
nanoparticles into Dendritic Flowers from aqueous solution;
submitted to J. Exp. Nanosci
3.
C Pettegrew, R Jayini, MR Islam (2009) Methanol containing
Transfer Buffer can be used multiple times in
Protein Electrotransfer;
accepted
J. Biomolecular Tech
4.
MR Islam,
S Puri, M Rodova, BS Magenheimer, RL Maser,
J P Calvet (2008) Activation of the Polycystic Kidney Disease-1 (PKD1)
Promoter by Retinoic Acid: involvement of Sp1; Am J
Physiol Renal Physiol 295: F1845-F1954
5.
Z Dong, D
Richardson, C Pelham, MR Islam (2008)
Rapid Synthesis of Silver Nanoparticles using a Household Microwave and
their characterization - a Simple Experiment for Nanoscience Laboratory; Chem.
Edu. 13: 240-243
6.
AS Baker, C
Pelham, Z Dong, MR Islam (2008) Probes
biotinylation by A-tailing and use in DNA-protein interactions;
American Biotech. Lab. 26(4): 26-27
7.
AS Baker, T
Jimenez, Z Dong,, P Ruth, MR Islam (2007) Purification of
Lysozyme from Hen Eggs using Sand;
Eur. J. Sci. Res. 16(3): 358-366
8.
MR Islam, GN Shah, WS Sly (2007) PCR-Based RFLP and Haplotype of the Most Common Mutation, L176F,
in the
b–glucuronidase Gene;
Genetic
Testing 11(1): 72-74
9.
R Islam, J Kite, AS Baker, A Ching Jr., MR Islam (2006) Affinity
Purification of Hen Egg Lysozyme using Sephadex G75;
Afr. J. Biotechnol. 5(20):
1902-1908
10.
S
Puri, M Rodova, MR Islam, BS Magenheimer, RL Maser, JP
Polycystic Kidney Disease-1 (PKD1)
Promoter; Biochem. Biophys. Research Communication
342: 1005-1013.
11.
M Rodova, MR Islam, KR Peterson, JP
intron in the
PKD1 gene; Mol Biol Evol 20: 1669-1674.
12.
I Schwartz, LR Silva, S Leistner, La Todeschini, MG Burin, MR Islam,
GN Shah, WS Sly,
R Giugliani (2003) Mucopolysachharidosis VII: clinical,
biochemical and molecular investigation of
a Brazilian family; Clin
Genet 64:172-175.
13.
in longest known
survivor of Mucopolysaccharidosis VII; Hum
Genet 112:190-194.
14. M
Rodova, MR Islam, RL Maser and JP Calvet (2002) The Polycystic Kidney
Disease-1
Promoter is a Target of the
beta-Catenin/T-cell Factor Pathway. J. Biol. Chem. 277:
29577-29583.
15. MR
Islam, M Rodova and JP Calvet (2002) A Fast and Efficient
Method of DNA fragment
isolation from Agarose Gels
without using Commercial Kits. Amer. Biotech. Lab. 20:18
16. S
Tomatsu, KO Orii, MR Islam, GN Shah, JH Grubb, K Sukegawa, Y Suzuki, T
Orii, N Kondo
and WS Sly (2002) Methylation
Patterns of Human b-glucuronidase Gene Locus:
Boundaries of
Methylation and General
Implications for Frequent Point Mutations at CpG Dinuclotides.
Genomics. 79: 363-375
17. MR
Islam, A Waheed, GN Shah,
of human egasyn nor C-terminal of b-glucuronidase is involved in their complex formation.
Arch. Biochem. Biophys. 372: 53-61
18. MR
Islam,
as the nucleophile and Glu451 as the acid-base residue; J. Biol. Chem. 274: 23451-23455
19. MR
Islam, P Strisciuglio, G Bonapace & WS Sly (1998)
Successful DNA-based prenatal diagnosis of
the 728+1 g-t mutation at the
exon 6-intron 6 junction in the carbonic
anhydrase II gene;
Prenat.
Diag. 19: 182-183
20. Y
Minami, MR Islam & G Funatsu (1998) Chemical modifications of
momordin-a and luffin-a, ribosome-inactivating proteins
from the seeds of Momordica
charantia and Luffa cylindrica: involvement of His140, Tyr165, and Lys231 in
the
protein-synthesis inhibitory activity; Biosci. Biotech. Biochem. (
21. MR
Islam, MPG
Arreola, Paul Wong,
b-glucuronidase
P408S, P415L allele in a northern
in
22. MR
Islam, GN Shah & WS Sly (1996) Enhanced translation of
rat b-glucuronidase cDNA is
conferred by 155 bp fragment of internal coding region; Arch. Biochem. Biophys. 333: 385-393
23. MR
Islam, R Vervoort, W Lissens, JJ Hoo, LA Valentino & WS
Sly (1996) b-glucuronidase
P408S, P415L mutations:
evidence that both mutations combine to produce a MPS type VII allele
in Mexican patients; Hum. Genet
98: 281-284
24. R
Vervoort, MR Islam, WS Sly, MT Zabot, WJ Kleijer, A Chabas, A
Fensom, EP Young,
I Liebaers & W Lissens (1996)
Molecular analysis of patients with b-glucuronidase
deficiency
presenting as Hydrops Fetalis or as
Early Mucopolysaccharidosis VII;
Am. J. Hum. Genet. 58:
457-471
25. R
Vervoort, MR Islam, WS Sly, A Chabas, J de Jong, I Liebaers & W
Lissens (1995)
A pseudodeficiency allele of the human b-glucuronidase gene; Am. J. Hum. Genet 57: 798-804
26. S
Yamada, S Tomatsu, WS Sly, MR Islam,
DA Wenger, S Fukuda, K Sukegawa and T Orii (1995)
Four novel mutations in
Mucopolysaccharidosis type VII including a unique base substitution in
exon 10 of the b-glucuronidase gene creates
a novel 5'-splice site; Hum. Mol.
Genet. 4: 651-655
27. MR
Islam, JH Grubb & WS Sly (1993) C-terminal processing of
human b-glucuronidase: the
propeptide is necessary for full
expression of catalytic activity, intracellular retention and proper
phosphorylation; J. Biol. Chem. 268: 22627-22633
28. G
Funatsu, MR Islam, Y Minami, SS Kung & M Kimura (1991) Conserved
amino acids in
ribosome-inactivating proteins from plants; Biochimie 73: 1157-1161
29. MR
Islam, SS Kung, Y Kimura & G Funatsu (1991) GlcNAc-Asn
structure in ribosome-
inactivating proteins from Luffa cylindrica and Phytolacca
Agric. Biol. Chem. (
30. MR
Islam, H Hirayama & G Funatsu (1991) Complete amino acid
sequence of luffin-b,
a ribosome-inactivating
protein from the Sponge gourd (Luffa
cylindrica) seeds;
Agric. Biol. Chem. (
31. MR
Islam, H Nishida & G Funatsu (1990) Complete amino acid
sequence of luffin-a,
a ribosome-inactivating
protein from the seeds of Sponge gourd (Luffa
cylindrica);
Agric. Biol. Chem. (
32. MR
Islam & G Funatsu (1988) Purification and
characterization of the constituent polypeptide
chains of Abrus precatorius agglutinin; Agric.
Biol. Chem. (
GRANTS
1. Applied Research Fund
(NWMSU), 2006-2007:
Project Title: A
non-radioactive method for DNA-protein analysis.Analysis.
PI: Rafiq Islam
($1,645)
2. National Institute of
Health (NIH), 2005-2009:
Project Title: MEKK1 down-regulates the PKD1 gene promoter by a
novel mechanism.
PI: Rafiq
Islam
($198,265)
4.
Federal Appropriations Fund
Project: “Instrumentations on
nanoscience”
PI: Barry Eichler, Co-PI: David Richardsion, Jeff
Thornsberry & Rafiq Islam ($1.18 m)
3.
Luminometer Grant, Turner BioSystems (
Project Title: Nuclear localization of MEKK1 and its role.
PI: Rafiq Islam
(A TD 20/20 luminometer ~ $5,200)
5.
Faculty Research Fund (NWMSU), 2000-2001:
Project Title:
Analysis of active component(s) present in a traditional drug for
treatment of Sarcoidosis in
PI: Rafiq Islam
($2,377)
6.
Applied Research Fund (NWMSU), 1999-2000:
Project Title:
Characterization of Powdery Mildew disease resistance factor(s) in Cucumis melo var. Sweet Thing hybrid and
Characterization of factor(s) present in Bitter
melon (Momordica charantia) that cause delay of tumor development in 69-2F
wap-ras mice.
PI: Dr.
Alex Ching Jr., Co-PI: Rafiq Islam
& David Smith ($10,000)
UNDERGRADUATE RESEARCH PRESENTATION
1. Chris
Pelham: “HFE promoter is regulated by IRF?” presented at
American Society of Biochemistry and Molecular Biology
(ASBMB) meeting in
2.
Chris Pelham: “Transcriptional Repression of HFE
promoter” presented at
(i)
Celebration of Quality meeting at Northwest (Mar 10, 2007) &
(ii) Missouri Academy of Science meeting
at
3.
Aaron Baker: “Biotin labeling of probes and
DNA-protein interactions” presented at
(i)
Celebration of Quality meeting at Northwest (Mar 10, 2007) &
(ii)
Missouri Academy of Science meeting at
4.
Zhen Dong: “A simple method for Ag nanoparticle
synmthesis” presented at
(i)
Celebration of Quality meeting at Northwest (Mar 10, 2007) &
(ii)
Missouri Academy of Science meeting at
5.
Andrew Swatek: “Silver nanoparticle synthesis and
self assembly into nanoflower” presented at
(i)
Celebration of Quality meeting at Northwest (Mar 10, 2007) &
(ii)
Missouri Academy of Science meeting at
6. Aaron Baker:
“Sand as a Matrix for Purification of Lysozyme from Hen Eggs” presented at
(i) Celebration of Quality at Northwest (Mar 11, 2006) &
(ii) Missouri Academy of Science Meeting at
7. Tamara Jimenez & Angela Rudolph: “Regulation of HFE Gene Promoter” presented at
(i) Celebration of Quality at Northwest (Mar 11, 2006) &
(ii) Missouri Academy of Science
Meeting at
8. Rizwana
Islam: “One step, Large-scale purification of Lysozyme from Hen Egg White”
presented at
(i)
Celebration 2002 Symposium at Northwest (Mar 12, 2005) &
(ii)
Missouri Academy of Science Meeting at
9.
Brian Schaffer : “ Binding effect of Bovine Serum Albumin with Cibacron blue” presented at
(i) Celebration 2002 Symposium at
Northwest (Mar 2, 2002) &
(ii)
Missouri Academy of Science Meeting at
10. Andrea Sigwing: “Metal Microbe Interactions: the Oxidation of Mn
by L. Discophora Sp-6” presented at
Missouri Academy of Science meeting at
11. L. Ndegwa: "Sarcoidosis
Tablet Analysis" presented at
(i) Celebration 2001 at
Northwest (Mar 31, 2001) &
(ii)
Department of
Chemistry/Physics, NWMSU (Apr 5, 2001)
12. U. Nwoye: "Gene for Powdery Mildew
Resistance in Korean Melons" presented at
(i) Alpha-Khi Regional Meeting at the University of Missouri-Columbia
(3/31-4/1, 2000), &
(ii) Celebration 2000 at
Northwest (Apr 15, 2000).
13. M. Nwoye,: "Isolation
of a Protein-synthesis inhibitory proteins from bitter melons" presented
at
(i) Alpha-Khi Regional
Meeting at the University of Missouri-Columbia (3/31-4/1, 2000), &
(ii) Celebration 2000 at
Northwest (Apr 15, 2000).
1. “Repression of Polycystic Kidney Disease-1 Promoter by
MEKK1 is mediated through p53” Poster presentation at
ASBMB (American Society of Biochemistry &
Molecular Biology) meeting, San Diego (May 5-9, 2008)
2. “Polycystic Kidney Disease-1 Promoter is activated by
Retinoic acid-involvement of Sp1” Poster presentation at
American Society of Nephrology meeting, San Diego (Oct
29-31, 2007)
3.“Polycystic Kidney Disease-1 Promoter
is activated by Retinoic acid-involvement of Sp1” poster presentation at
American Society of Biochemistry and Molecular Biology
meeting in Washington DC (Apr 27-May 2, 2007)
4. “Polycystic
Kidney Disease-1 Promoter is downregulated by MEKK1-a novel mechanism” University
of Kansas Medical Center (Mar 19, 2007)
5. “The Human PKD1 Promoter is Regulated by Ets Family
Transcription Factors”
at American Society of Nephrology in
Pennsylvania (Nov 1- 4, 2002).
6. “Polycystic Kidney Disease Gene, Polycystin-1, is a
target for beta-catenin/TCF pathway”.
Poster presentation at
FASEB Summer Research Conferences in Colorado (Jul 7-12, 2001), and Chromatin and Transcription Meeting in
Cold Spring Harbor Laboratory (Aug 29-Sep 2, 2001)
7.
“A Fast and Efficient Method of DNA fragment isolation from Agarose Gels
without using Commercial Kits” presented at
Missouri Academy of Science meeting at Springfield,
MO (Apr 19-20, 2002)